Minimum wage setting and standards of fairness

We examine the setting of minimum wages, arguing that they can best be understood as a reflection of voters' notions of fairness. We arrive at this conclusion through an empirical investigation of the implications of three models, considered in the context of policy setting by sub-units in a federation: a competing interests group model; a constrained altruism model; and a fairness based model. In the latter model, voters are interested in banning what they view to be unfair transactions, with the notion of fairness based on comparisons to the "going" unskilled wage. We use data on minimum wages set in the ten Canadian provinces from 1969 to 2005 to carry out the investigation. A key implication of the models that is borne out in the data is that minimum wages should be set as a positive function of the location of the unskilled wage distribution. Together, the results indicate that minimum wages are set according to a "fairness" standard and that this may exacerbate movements in inequality.

Automated tagging of environmental data using a novel SKOS formatted environmental thesaurus

There is increasing need to use the widest range of data to address issues of environmental management and change, which is reflected in increasing emphasis from government funding agencies for better management and access to environmental data. Bringing together different environmental datasets to confidently enable integrated analysis requires reference to common standards and definitions, which are frequently lacking in environmental data, due to the broad subject area and lack of metadata. Automatic inclusion within datasets of controlled vocabulary concepts from publicly available standard vocabularies facilitates accurate annotation and promotes efficiency of metadata creation. To this end, we have developed a thesaurus capable of describing environmental chemistry datasets. We demonstrate a novel method for tagging datasets, via insertion of this thesaurus into a Laboratory Information Management System, enabling automated tagging of data, thus promoting semantic interoperability between tagged data resources. Being web available, and formatted using the Simple Knowledge Organisation System (SKOS) semantic standard, this thesaurus is capable of providing links both to and from other relevant thesauri, thus facilitating a linked data approach. Future developments will see extension of the thesaurus by the user community, in terms of both concepts included and links to externally hosted vocabularies. By employing a Linked Open Data approach, we anticipate that Web-based tools will be able to use concepts from the thesaurus to discover and link data to other information sources, including use in national assessment of the extent and condition of environmental resources

Meeting the challenge of environmental data publication: an operational infrastructure and workflow for publishing data

Here we describe the defined workflow and its supporting infrastructure, which are used by the Natural Environment Research Council’s (NERC) Environmental Information Data Centre (EIDC) (http://​eidc.​ceh.​ac.​uk/​) to enable publication of environmental data in the fields of ecology and hydrology. The methods employed and issues discussed are also relevant to publication in other domains. By utilising a clearly defined workflow for data publication, we operate a fully auditable, quality controlled series of steps permitting publication of environmental data. The described methodology meets the needs of both data producers and data users, whose requirements are not always aligned. A stable, logically created infrastructure supporting data publication allows the process to occur in a well-managed and secure fashion, while remaining flexible enough to deal with a range of data types and user requirements. We discuss the primary issues arising from data publication, and describe how many of them have been resolved by the methods we have employed, with demonstrable results. In conclusion, we expand on future directions we wish to develop to aid data publication by both solving problems for data generators and improving the end-user experience

Implementation of a workflow for publishing citeable environmental data: successes, challenges and opportunities from a data centre perspective

In recent years, the development and implementation of a robust way to cite data have encouraged many previously sceptical environmental researchers to publish the data they create, thus ensuring that more data than ever are now open and available for re-use within and between research communities. Here, we describe a workflow for publishing citeable data in the context of the environmental sciences—an area spanning many domains and generating a vast array of heterogeneous data products. The processes and tools we have developed have enabled rapid publication of quality data products including datasets, models and model outputs which can be accessed, re-used and subsequently cited. However, there are still many challenges that need to be addressed before researchers in the environmental sciences fully accept the notion that datasets are valued outputs and time should be spent in properly describing, storing and citing them. Here, we identify current challenges such as citation of dynamic datasets and issues of recording and presenting citation metrics. In conclusion, whilst data centres may have the infrastructure, tools, resources and processes available to publish citeable datasets, further work is required before large-scale uptake of the services offered is achieved. We believe that once current challenges are met, data resources will be viewed similarly to journal publications as valued outputs in a researcher’s portfolio, and therefore both the quality and quantity of data published will increase

Is Drotrecogin alfa (activated) for adults with severe sepsis, cost-effective in routine clinical practice?

INTRODUCTION: Previous cost-effectiveness analyses (CEA) reported that Drotrecogin alfa (DrotAA) is cost-effective based on a Phase III clinical trial (PROWESS). There is little evidence on whether DrotAA is cost-effective in routine clinical practice. We assessed whether DrotAA is cost-effective in routine practice for adult patients with severe sepsis and multiple organ systems failing. METHODS: This CEA used data from a prospective cohort study that compared DrotAA versus no DrotAA (control) for severe sepsis patients with multiple organ systems failing admitted to critical care units in England, Wales, and Northern Ireland. The cohort study used case-mix and mortality data from a national audit, linked with a separate audit of DrotAA infusions. Re-admissions to critical care and corresponding mortality were recorded for four years. Patients receiving DrotAA (n = 1,076) were matched to controls (n = 1,650) with a propensity score (Pscore), and Genetic Matching (GenMatch). The CEA projected long-term survival to report lifetime incremental costs per quality-adjusted life year (QALY) overall, and for subgroups with two or three to five organ systems failing at baseline. RESULTS: The incremental costs per QALY for DrotAA were £30,000 overall, and £16,000 for the subgroups with three to five organ systems failing. For patients with two organ systems failing, DrotAA resulted in an average loss of one QALY at an incremental cost of £15,000. When the subgroup with two organ systems was restricted to patients receiving DrotAA within 24 hours, DrotAA led to a gain of 1.2 QALYs at a cost per QALY of £11,000. The results were robust to other assumptions including the approach taken to projecting long-term outcomes. CONCLUSIONS: DrotAA is cost-effective in routine practice for severe sepsis patients with three to five organ systems failing. For patients with two organ systems failing, this study could not provide unequivocal evidence on the cost-effectiveness of DrotAA

Spatio-temporal expression patterns of Arabidopsis thaliana and Medicago truncatula defensin-like genes

Plant genomes contain several hundred defensin-like (DEFL) genes that encode short cysteine-rich proteins resembling defensins, which are well known antimicrobial polypeptides. Little is known about the expression patterns or functions of many DEFLs because most were discovered recently and hence are not well represented on standard microarrays. We designed a custom Affymetrix chip consisting of probe sets for 317 and 684 DEFLs from Arabidopsis thaliana and Medicago truncatula, respectively for cataloging DEFL expression in a variety of plant organs at different developmental stages and during symbiotic and pathogenic associations. The microarray analysis provided evidence for the transcription of 71% and 90% of the DEFLs identified in Arabidopsis and Medicago, respectively, including many of the recently annotated DEFL genes that previously lacked expression information. Both model plants contain a subset of DEFLs specifically expressed in seeds or fruits. A few DEFLs, including some plant defensins, were significantly up-regulated in Arabidopsis leaves inoculated with Alternaria brassicicola or Pseudomonas syringae pathogens. Among these, some were dependent on jasmonic acid signaling or were associated with specific types of immune responses. There were notable differences in DEFL gene expression patterns between Arabidopsis and Medicago, as the majority of Arabidopsis DEFLs were expressed in inflorescences, while only a few exhibited root-enhanced expression. By contrast, Medicago DEFLs were most prominently expressed in nitrogen-fixing root nodules. Thus, our data document salient differences in DEFL temporal and spatial expression between Arabidopsis and Medicago, suggesting distinct signaling routes and distinct roles for these proteins in the two plant species

Why do plasmids manipulate the expression of bacterial phenotypes?

From The Royal Society via Jisc Publications RouterHistory: received 2021-03-17, accepted 2021-07-09, pub-electronic 2021-11-29, pub-print 2022-01-17Article version: VoRPublication status: PublishedFunder: Natural Environment Research Council; Id: http://dx.doi.org/10.13039/501100000270; Grant(s): NE/R008825/1Funder: Biotechnology and Biological Sciences Research Council; Id: http://dx.doi.org/10.13039/501100000268; Grant(s): BB/R014884/1Conjugative plasmids play an important role in bacterial evolution by transferring niche-adaptive traits between lineages, thus driving adaptation and genome diversification. It is increasingly clear, however, that in addition to this evolutionary role, plasmids also manipulate the expression of a broad range of bacterial phenotypes. In this review, we argue that the effects that plasmids have on the expression of bacterial phenotypes may often represent plasmid adaptations, rather than mere deleterious side effects. We begin by summarizing findings from untargeted omics analyses, which give a picture of the global effects of plasmid acquisition on host cells. Thereafter, because many plasmids are capable of both vertical and horizontal transmission, we distinguish plasmid-mediated phenotypic effects into two main classes based upon their potential fitness benefit to plasmids: (i) those that promote the competitiveness of the host cell in a given niche and thereby increase plasmid vertical transmission, and (ii) those that promote plasmid conjugation and thereby increase plasmid horizontal transmission. Far from being mere vehicles for gene exchange, we propose that plasmids often act as sophisticated genetic parasites capable of manipulating their bacterial hosts for their own benefit. This article is part of the theme issue ‘The secret lives of microbial mobile genetic elements’

Synthesis and Catalytic Activity of (3,4-Diphenylcyclopentadienone)Iron Tricarbonyl Compounds in Transfer Hydrogenations and Dehydrogenations

Four (3,4-diphenylcyclopentadienone)iron tricarbonyl compounds were synthesized, and their activities in transfer hydrogenations of carbonyl compounds and transfer dehydrogenations of alcohols were explored and compared to those of the well-established [2,5-(SiMe3)2-3,4-(CH2)4(η4-C4C═O)]Fe(CO)3 (3). A new compound, [2,5-bis(3,5-dimethylphenyl)-3,4-diphenylcyclopentadienone]iron tricarbonyl (7), was the most active catalyst in both transfer hydrogenations and dehydrogenations, and compound 3 was the least active catalyst in transfer hydrogenations. Evidence was found for product inhibition of both 3 and 7 in a transfer dehydrogenation reaction, with the activity of 3 being more heavily affected. A monomeric iron hydride derived from 7 was spectroscopically observed during a transfer hydrogenation, and no diiron bridging hydrides were found under reductive or oxidative conditions. Initial results in the transfer hydrogenation of N-benzylideneaniline showed that 3 was a significantly less active catalyst in comparison to the (3,4-diphenylcyclopentadienone)iron tricarbonyl compounds